小鼠GV期卵母细胞的提取及其核质比分析.doc
上传者：小雄
2022-05-25 14:28:28上传
DOC文件
87 KB
小鼠GV期卵母细胞的提取及其核质比分析.doc小鼠GV期卵母细胞的
提取及其核质比分析
作者：洪灯，符皎荣,张彦慧，钟南田,郑小桃
单位：海南医学院组织学与胚胎学教研室，海南海口
【摘要】目的：提取小鼠GV (germinal vesicle, GV)期卵母 细胞并分析其核质比。方法：用挤压法从卵巢中分离获得GV期 卵母细胞，放入DEPC-NS液体快速清除颗粒细胞，获得GV期卵 母细胞裸卵，并计算其核质比。结果：用挤压法分离获得的GV 期卵母细胞有一完整球形核，核膜明显，含有单个折光的核仁， 胞浆中心稍变黑并呈颗粒状，共51枚，测得其卵径为 (40.940± 3.341)μm , 核 径 为
(15.020± 2.236)μm , 其 核 质 比 为
0. 054±0. 019o结论：用挤压法加DEPC-NS液体可快速破 坏颗粒细胞之间的连接，便于迅速获得GV期卵母细胞裸卵；小鼠 GV期卵母细胞的核质比为0. 054±0. 019,与其它物种同 时相核质比在相同范围内，提示核质比是卵母细胞发育成熟的重 要指标。
【关键词】小鼠;GV期卵母细胞;挤压法;核质比
[ABSTRACT] Objective： To extract the GV-stage oocytes of mouse and analyzed its the nucleus-plasma ratio. Methods
： GV-stage oocytes were extracted and isolated by extrusion. Granulosa cells were quickly removed in DEPC-NS liquid and denuded oocytes were obtained. The nucleus-plasma ratio of the denuded oocytes was caculated. Results： The GV-stage oocytes had complete spherical nucleus with obvious nuclear membrane, and a single refractive nucleolus. Its cytoplasm center was a little black and in granular. A total of 51 GV-stage eggs were obtained , which were (40.940±3.341) &mu:m in diameter and (15. 020±2. 236) &mu:m in core diameter, and the nucleus-plasma ratio was 0.054 ± 0.019. Conclusions： The extrusion method and DEPC-NS liquid can rapidly destruct the connection between the granulosa cells, in which the denuded GV-stage oocyte
提取及其核质比分析
作者：洪灯，符皎荣,张彦慧，钟南田,郑小桃
单位：海南医学院组织学与胚胎学教研室，海南海口
【摘要】目的：提取小鼠GV (germinal vesicle, GV)期卵母 细胞并分析其核质比。方法：用挤压法从卵巢中分离获得GV期 卵母细胞，放入DEPC-NS液体快速清除颗粒细胞，获得GV期卵 母细胞裸卵，并计算其核质比。结果：用挤压法分离获得的GV 期卵母细胞有一完整球形核，核膜明显，含有单个折光的核仁， 胞浆中心稍变黑并呈颗粒状，共51枚，测得其卵径为 (40.940± 3.341)μm , 核 径 为
(15.020± 2.236)μm , 其 核 质 比 为
0. 054±0. 019o结论：用挤压法加DEPC-NS液体可快速破 坏颗粒细胞之间的连接，便于迅速获得GV期卵母细胞裸卵；小鼠 GV期卵母细胞的核质比为0. 054±0. 019,与其它物种同 时相核质比在相同范围内，提示核质比是卵母细胞发育成熟的重 要指标。
【关键词】小鼠;GV期卵母细胞;挤压法;核质比
[ABSTRACT] Objective： To extract the GV-stage oocytes of mouse and analyzed its the nucleus-plasma ratio. Methods
： GV-stage oocytes were extracted and isolated by extrusion. Granulosa cells were quickly removed in DEPC-NS liquid and denuded oocytes were obtained. The nucleus-plasma ratio of the denuded oocytes was caculated. Results： The GV-stage oocytes had complete spherical nucleus with obvious nuclear membrane, and a single refractive nucleolus. Its cytoplasm center was a little black and in granular. A total of 51 GV-stage eggs were obtained , which were (40.940±3.341) &mu:m in diameter and (15. 020±2. 236) &mu:m in core diameter, and the nucleus-plasma ratio was 0.054 ± 0.019. Conclusions： The extrusion method and DEPC-NS liquid can rapidly destruct the connection between the granulosa cells, in which the denuded GV-stage oocyte
